Loading...
This record has been deleted. Since it is linked to a published record, it is available here for reference purposes only.
Click here to view the deleted recordThis document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Black nightshade modified for reduced production of endogenous proteinase inhibitors
EN
S06-336, S06-353, S06-354 and S06-356
No
-
Organization:Max Planck Institute for Chemical Ecology (MPICE)Academic or research instituteHans-Knöll-Strasse 8Jena,
07745, GermanyPhone: +49 3641 57-0,Fax: +49 3641 57-1002,Email: info@ice.mpg.de,Website: https://www.ice.mpg.de,
The genes pin1 and pin2b from Solanum nigrum each codes for a type II serine proteinase inhibitors (PIN), which block the proteolytic activity of trypsin and chymotrypsin. Plants produce proteinase inhibitors as a strategy to protect themselves against the damage caused by herbivorous insects.
In the genetically modified plants S06-336, S06-353, S06-354 and S06-356 the synthesis of two of the plant’s native proteinase inhibitors, PIN1 and PIN2b, is reduced by post-transcriptional gene silencing. For this purpose, an RNAi construct consisting of fragments of the pin1 and pin2b genes from S. nigrum was developed.
As a result of the genetic modification, the targeted reduction of the production of the PIN2b and PIN1 proteins is expected to reduce the genetically modified plant’s defences against herbivory.
EN
In the genetically modified plants S06-336, S06-353, S06-354 and S06-356 the synthesis of two of the plant’s native proteinase inhibitors, PIN1 and PIN2b, is reduced by post-transcriptional gene silencing. For this purpose, an RNAi construct consisting of fragments of the pin1 and pin2b genes from S. nigrum was developed.
As a result of the genetic modification, the targeted reduction of the production of the PIN2b and PIN1 proteins is expected to reduce the genetically modified plant’s defences against herbivory.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
-
BCH-ORGA-SCBD-47832-5 Organism Solanum nigrum (Black nightshade, SOLNI)Crops
Cultivar/breeding line: Sn 30
EN
-
Black Nightshade with reduced pathogenesis-related protein 1S synthesis.| Max Planck Institute for Chemical Ecology(MPICE) | Resistance to antibiotics (Hygromycin), Resistance to diseases and pests
-
Black Nightshade with reduced pathogenesis-related protein 1S synthesis| Max Planck Institute for Chemical Ecology(MPICE) | Resistance to antibiotics (Hygromycin), Resistance to diseases and pests
-
Black nightshade modified for reduced production of endogenous prosystemin| Max Planck Institute for Chemical Ecology(MPICE) | Resistance to antibiotics (Hygromycin), Resistance to diseases and pests
pSOL3PIN12
EN
- Agrobacterium-mediated DNA transfer
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
-
BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
-
BCH-GENE-SCBD-110663-1 Potato type II proteinase inhibitor, PIN2B | Solanum nigrum (Black nightshade, SOLNI)Protein coding sequence | Resistance to diseases and pests (Insects)
-
BCH-GENE-SCBD-110662-2 Potato type II proteinase inhibitor, PINI | Solanum nigrum (Black nightshade, SOLNI)Protein coding sequence | Resistance to diseases and pests (Insects)
-
BCH-GENE-SCBD-103123-6 Pyruvate orthophosphate dikinase, Intron 3 | Flaveria trinervia (Clustered Yellowtops, speedyweed, flaveria, yellow twinstem)Intron
-
BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
-
BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
-
BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
-
BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
Fragments of the proteinase inhibitor genes pin1 and pin2b derived from S. nigrum were linked in a chimeric fragment of 728 bp. The plasmid pSOL3-PIN12 used for transformation includes a construct in which two of these 728 bp fragments were arranged in sense and antisense orientation, separated by a spacer. The 785 bp long third intron of the pyruvate orthophosphate dikinase gene (pdk i3) of Flaveria trinervia, of which splicing activity is suspected, was used as a spacer. The expression is controlled by the promoter and the termination signal of the 35S gene of cauliflower mosaic virus (CaMV).
The hygromycin phosphotransferase gene (hptII) of Escherichia coli expressed under the control of the promoter and termination signal of the nopaline synthase gene of Agrobacterium tumefaciens was used as a selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.
EN
The hygromycin phosphotransferase gene (hptII) of Escherichia coli expressed under the control of the promoter and termination signal of the nopaline synthase gene of Agrobacterium tumefaciens was used as a selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.
EN
- Research
EN
EN
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Living Modified Organism | Related LMO(s) | 3 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
